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Lorem Ipsum
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It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages.
*Layout of question 8 and 9 are the same
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Question 7: What Cas enzyme, with which PAM site, is suitable? Design gRNAs (single target or multi-target) next to the PAM site and design amplicons spanning the gRNA.
Guidelines for sgRNA design (Schindele et al., 2020).
Software based on:
- What algorithm to predict specificity/efficiency.
- Possibilities to take into account allelic diversity and sequence similarity with non-desired target sequences.
- When possible, test of the sgRNAs in a protoplast system is recommended.
gRNA and primer design in geneious1,2
gRNA design in CRISPOR and FLASHFRY1,2
PlantPegDesigner tool3
Tool for HDR4
Lorem Ipsum
Lorem ipsum is simply dummy text of the printing and typesetting industry. Lorem Ipsum has been the industry’s standard dummy text ever since the 1500s.
It has survived not only five centuries, but also the leap into electronic typesetting, remaining essentially unchanged. It was popularised in the 1960s with the release of Letraset sheets containing Lorem Ipsum passages.
*Layout of question 7 and 10 are the same
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